| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7620613 | Journal of Food Composition and Analysis | 2015 | 9 Pages |
Abstract
A method for the simultaneous determination of carotenoids, tocopherols, tocotrienols, alk(en)ylresorcinols, and steryl ferulates from whole grain wheat flour was developed and validated using HPLC-DAD/FLD-MSn. After comparing various solvents and extraction techniques, samples were extracted with acetone without previous saponification. Extraction recoveries ranged from 93.2 to 101.6% for carotenoids, tocopherols, and steryl ferulates, while 83.4-99.4% were determined for alkylresorcinols. The inter-day CV for all analytes was below 5%. HPLC separation was compared on porous particle C18- and C30- as well as a core-shell particle C18-column, achieving optimum separation on the latter within 40Â min after gradient optimization. The limit of detection (LOD) ranged from 0.03 to 20.41Â ng on column for lutein and tricosylresorcinol, respectively. Further validation parameters are reported in detail. HPLC-MSn supported the identification of minor compounds, when standards were unavailable. This method is proved to be suitable for high-throughput screening of a large number of lipophilic compounds in wheat.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jochen U. Ziegler, Ralf M. Schweiggert, Reinhold Carle,