Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7626417 | Journal of Pharmaceutical and Biomedical Analysis | 2018 | 19 Pages |
Abstract
Oxaliplatin is a platinum compound that is frequently prescribed for the chemotherapeutic treatment of colorectal cancer. In tumor cells, cellular uptake is the first step of oxaliplatin action. Cellular accumulation of oxaliplatin is considered to play an important role in anti-cancer efficacy. However, limited information about cellular accumulation of intact oxaliplatin is available. In this study, a sensitive hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) approach for the quantification of oxaliplatin in cells was developed and validated. The method allowed for a rapid and simple determination of intact oxaliplatin in cell lysate. The retention time of oxaliplatin was 3.04â¯min, which was achieved by applying a chromatographic gradient elution of 5â¯min. The lower limit of quantification (LLOQ) was 2â¯ng/mL and the analytical range of oxaliplatin was linear between 2-200â¯ng/mL. The intra-day precision and inter-day precision (RSD (relative standard deviation)) ranged from 0.52 to 7.89%, and the accuracy (RE (relative error)) was withinâ¯Â±â¯4.5%. Matrix effects and recovery were acceptable. The method was successfully used for the determination of intact oxaliplatin uptake by HCT-116 colon cancer cells. Thus, our findings may prospectively support a celluar pharmacokinetic study and low concentration measurement of intact oxaliplatin in the clinic.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Zhiying Qin, Guanghui Ren, Qi Liu, Xiaoyu Lu, Qing Zhang, Ali Fan, Yang Lu, Ning Li, Xijng Chen, Di Zhao,