Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7626979 | Journal of Pharmaceutical and Biomedical Analysis | 2018 | 33 Pages |
Abstract
Cladrin, an isoflavone is a major bioactive constituent found in stem bark of Butea monosperma with remarkable osteogenic activity. A speedy and sensitive UPLC coupled tandem mass spectrometry (UPLC-MS/MS) method was developed, validated and successfully applied to bioavailability, blood partitioning, plasma protein binding, intravenous and multiple-dose oral pharmacokinetics of cladrin in rats. Separation was done on C18 column (5.0â¯Î¼m, 4.6â¯Ãâ¯50â¯mm) using mobile phase containing acetonitrile and 0.10% formic acid in the ratio of 65:35 (v/v) with 0.60â¯mL/min flow rate. The method was highly sensitive and has a short run time of 2.50â¯min with an excellent linearity (R2â¯>â¯0.99) in the range of 0.20-200â¯Î¼g/L. Absolute bioavailability was found to be 16.58, 19.04 and 6.76% at oral doses of 5, 10, and 20â¯mg/Kg, respectively. Cladrin was rapidly absorbed (Tmax 3.0â¯h) with a high apparent volume of distribution (15.03â¯Â±â¯1.79L/Kg), high clearance (2.27â¯Â±â¯0.30L/h/Kg) and high plasma protein binding. The present study is a first comprehensive in-vitro as well as the in-vivo preclinical pharmacokinetic report of cladrin giving insights about its drug-likeness and further development as a potential therapeutic agent.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mamunur Rashid, Sandeep K. Singh, Mohd Yaseen Malik, Sadaf Jahan, Swati Chaturvedi, Isha Taneja, Kanumuri Sivarama Raju, Zaiba Naseem, J.R. Gayen, Muhammad Wahajuddin,