Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7627509 | Journal of Pharmaceutical and Biomedical Analysis | 2018 | 19 Pages |
Abstract
The investigation of the possible conversion of cannabidiol (CBD) into Î9-tetrahydrocannabinol (THC) in vivo after oral administration of CBD is reported herein since recent publications suggested a rapid conversion in simulated gastric fluid. To this end, single high dose of CBD (50Â mg/kg) was administered orally to rats and their blood was collected after 3 and 6Â h. A highly sensitive and selective LC-MS/MS method was developed and fully validated in compliance with the Scientific Working Group of Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. This method also involved the optimization of cannabinoids and their metabolites extraction in order to remove co-eluting phospholipids and increase the sensitivity of the MS detection. Neither THC nor its metabolites were detected in rat whole blood after 3 or 6Â h from CBD administration. After oral administration, the amount of CBD dissolved in olive oil was higher than that absorbed from an ethanolic solution. This could be explained by the protection of lipid excipients towards CBD from acidic gastric juice.
Related Topics
Physical Sciences and Engineering
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Authors
Federica Palazzoli, Cinzia Citti, Manuela Licata, Antonietta Vilella, Letizia Manca, Michele Zoli, Maria Angela Vandelli, Flavio Forni, Giuseppe Cannazza,