Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7627555 | Journal of Pharmaceutical and Biomedical Analysis | 2018 | 15 Pages |
Abstract
Since ancient times bee pollen has been considered a good source of bioactive substances and energy. Taking into account the current demand for healthy and natural foods, it is not surprising that bee pollen has been attracting commercial interest in recent years, making it one of the most widely consumed food supplements. It has been extensively reported that bee pollen contains several health-promoting compounds, such as proteins, amino acids, lipids, phenolic compounds, vitamins or minerals. Thus, this study aims to give an overview of the extraction and determination techniques of several of the above-mentioned compounds which have been published in the last few years (2011-2017). The design of the study is in accordance with the different families of bioactive compounds, and the extraction procedures together with the analytical techniques employed and their determination are discussed. A list of some of the most relevant applications is provided for each category, including a brief summary of the experimental conditions. The references included will provide the reader with a comprehensive overview of and insight into the analysis of bioactive compounds from bee pollen.
Keywords
FCMSDSTrpHClNaOHMALDI-TOFDPPHBHTSFEAASPDAMECSPEICPSSADTTTPCECDOPABCAUHPLCDADTFCNIRSSFNIEFPAGEMPAFAASBPEFLMAlCl3AAAFFAsFLDKOHTTMNPLCTRISNaNO2HNO3HCLO4GVMNH2RPLCo-phthaldialdehydeOpen column chromatographyAl(NO3)3Aluminium nitratePITCTSPPdetermination methodsIproDNS1,1-diphenyl-2-picrylhydrazyl1,4-dithiothreitol2-mercaptoethanolAesFPEMS/MSHydrogen peroxidePhotodiode array detectordiode array detectorAmperometric detectorElectrochemical detectorRefractive index detectorflame ionization detectorAminoFree amino acidsEDTAEthylenediaminetetraacetic acidSupercritical fluid extractionSolid phase extractionextraction techniquesSterols3,5-dinitrosalicylic acid5-Sulfosalicylic acidMetaphosphoric acidhydrochloric acidPerchloric acidtotal amino acidsFree fatty acidsfatty acids methyl esterspolyacrylamide gel electrophoresisCapillary electrophoresisOESbicinchoninic acidElemental analyzerBioactive compoundsaluminium trichlorideTris(hydroxymethyl)aminomethaneTryptophanisoelectric focusingridFIDTAAsCarbon dioxideFluorimetric methodGravimetric methodsodium dodecyl sulfateSulforaphaneFAMEsAtomic emission spectrometryOptical emission spectrometryAtomic absorption spectroscopyMass spectrometryTandem mass spectrometryOccBABfluorescence detectorPhenylisothiocyanateLemTotal phenolic contenttotal protein contentMatrix-Assisted Laser Desorption/Ionization Time-of-Flightnot specifiedtotal anthocyanin contentTotal flavonoid contentFood supplementsNear infra-red Spectroscopytotal nitrogenSodium nitriteNitric acidH2O2butylated hydroxytoluenesodium hydroxidePotassium Hydroxideinductively coupled plasmaReducing sugarCO2Ultra-high performance liquid chromatographyreverse-phase liquid chromatographyGas chromatographyTotal lipidsTotal carotenoidsKjeldahlBee pollenGlucosinolates
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ana M. Ares, Silvia Valverde, José L. Bernal, MarÃa J. Nozal, José Bernal,