Probing the interaction of cefodizime with human serum albumin using multi-spectroscopic and molecular docking techniques

- The interaction of CEF and HSA was studied by multi-spectroscopic and molecular docking techniques.
- The binding of CEF with HSA was not only a static quenching procedure but also driven mainly by hydrogen bonds and van der Waals force.
- Binding constant, number of binding site and binding distance were calculated.
- CEF changed the conformation and secondary structure of HSA.
- CEF bound to hydrophobic pocket (site I, subdomain IIA) of HSA.