High performance liquid chromatographic determination of ultra traces of two tricyclic antidepressant drugs imipramine and trimipramine in urine samples after their dispersive liquid-liquid microextraction coupled with response surface optimization

- Design of a dispersive liquid-liquid microextraction-HPLC method for assay of antidepressants in urine.
- Use of response surface methodology for multivariate optimization of DLLME conditions.
- Method development and validation for assay of imipramine and trimipramine in urine samples.
- Enrichment factor, linear range and limit of detection of 161.7-186.7, 5-100 ng mL−1 and 0.6 ng mL−1, respectively.
- Possibility to determine the residual of drugs four days after using a 30 mg dosage in urine samples.