| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7630773 | Journal of Pharmaceutical and Biomedical Analysis | 2014 | 6 Pages |
Abstract
- A UT-7/epo cell line stably transfected with luciferase gene under the control of SIE and GAS promoter was constructed.
- A reporter gene assay for potency determination of rHuEPO was established and the procedure was optimized.
- The reporter gene assay was validated for its precision, accuracy, specificity and robustness.
- The consistency of reporter gene assay with reticulocyte assay was evaluated by Bland-Altman analysis.
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Authors
Yushuai Yang, Yong Zhou, Lei Yu, Xiang Li, Xinchang Shi, Xi Qin, Chunming Rao, Junzhi Wang,