| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7630873 | Journal of Pharmaceutical and Biomedical Analysis | 2014 | 7 Pages |
Abstract
- A cobalt albumin binding (CAB) assay was developed with a reaction buffer (50Â mM NaH2PO4, pH 7.4).
- Dithiothreitol (DTT) concentration and minimal sample volume were set for optimal reaction.
- It was confirmed that structural changes of human serum albumin (HSA) were detectable by CAB assay.
- CAB assay could detect conformational changes of HSA induced by various stimulants.
- CAB assay has the potential to be used for quality control of albumin injections.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Eunyoung Lee, Ji-Eun Eom, Kyung-Hwa Jeon, Tae Hee Kim, Eunnam Kim, Gil-Ja Jhon, Youngjoo Kwon,