| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7630977 | Journal of Pharmaceutical and Biomedical Analysis | 2014 | 8 Pages |
Abstract
- Development and validation of a simple, fast and reliable method for estimation of glucocerebrosidase activity based on high-performance liquid chromatography.
- The residual glucocerebrosidase activity in peripheral monocytes could be a reflection of glucocerebrosidase quantity in targets organs.
- Significant differences between healthy controls and patients were observed, so this suggests that discerning between them and providing an approach to enzyme dosage optimization is feasible.
- Monitoring of glucocerebrosidase in may be a new marker to drive dose selection and early clinical decision making of enzyme replacement therapy.
Keywords
ACNGBAFACSPNPImgGlcCerEMATRISFDAMLRPNP-Glc4-nitrophenyl-β-d-glucopyranosideHPLC–UVp-Nitrophenoltris-(hydroxymethyl)-aminomethaneβ-glucocerebrosidaseenzyme replacement therapyEuropean Medicines agencyEDTAAcetonitrileImigluceraseGaucher diseaseMultiple linear regressionEnzyme activityfluorescence-activated cell sorterERTunitHigh performance liquid chromatography with ultraviolet detectionHPLCGlucocerebroside
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
E. Gras Colomer, M.A. MartÃnez Gómez, A. González Álvarez, M. Climente MartÃ, P. León Moreno, M. Fernández Zarzoso, N.V. Jiménez-Torres,