Absolute quantification of UGT1A1 in various tissues and cell lines using isotope label-free UPLC-MS/MS method determines its turnover number and correlates with its glucuronidation activities

- Develop an isotope-free UPLC-MS method to absolutely quantify UGT1A1.
- Validate the accuracy of the method with respect to accuracy and precision.
- Corroborate the accuracy of this method using a standard addition approach.
- Demonstrate the correlation between UGT1A1 level and UGT1A1 activity.
- Determine the UGT1A1 turnover number in various cell lines using estradiol.