Liquid chromatographic determination of CPZEN-45, a novel anti-tubercular drug, in biological samples

- We developed an efficient method (>96%) to extract CPZEN-45 from biological samples.
- We validated an HPLC method to quantify CPZEN-45 in plasma, lung and spleen tissues.
- The HPLC method was validated as per the FDA guidelines.
- The limit of detection was 0.05 μg/ml and the limit of quantitation was 0.29 μg/ml.
- This method can detect CPZEN-45 concentrations that are 5- to 21-fold smaller than MIC.