Quantitative capillary zone electrophoresis method for the precise determination of charge differences arising from the manufacture of heparan-N-sulfatase

Article ID	Journal	Published Year	Pages	File Type
7631487	Journal of Pharmaceutical and Biomedical Analysis	2013	7 Pages	PDF

Abstract

- Capillary zone electrophoresis method for resolving isoforms of heparan-N-sulfatase.
- Separation carried out with bare fused silica capillary and 25Â mM Tris, pH 8.0 buffer.
- Method allows separation/integration of 14 peaks (glycoforms) to monitor purity/stability.
- Glycoforms arise from differing amounts of sialic acid and mannose-6-phosphate.
- Method can discriminate differences in charge across batches of heparan-N-sulfatase.

Keywords

SDS RSD Diglycine HEPES Charge variant CZE HNS HPAE-PAD EOF cIEF M6P BGE CITP NP40 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid Capillary isotachophoresis EDTA Ethylenediaminetetraacetic acid Capillary zone electrophoresis relative standard deviation Isoform Tris(hydroxymethyl)aminomethane Tris(hydroxymethyl)aminomethane hydrochloride Tris–HCl capillary isoelectric focusing Electroosmotic flow sodium dodecyl sulphate mannose-6-phosphate Heterogeneity Glycosylation