Simultaneous determination of harmine, harmaline and their metabolites harmol and harmalol in beagle dog plasma by UPLC-ESI-MS/MS and its application to a pharmacokinetic study

- Harmine (HAR) and harmaline (HAL) are potential in AD, PD, and depression.
- HAR and HAL were demethylated to form harmol (HOL) and harmalol (HAM).
- HAR, HAL, HOL, and HAM were simultaneously determined by UPLC-ESI-MS/MS.
- The low limits of quantification for HAR, HAL, HOL, and HAM were all 1.00 ng/ml.
- The pharmacokinetic study in beagle dogs after intravenously dose of HAR and HAL.