Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7631843 | Journal of Pharmaceutical and Biomedical Analysis | 2013 | 6 Pages |
Abstract
- We developed an HPLC method for determination arachidonic acid metabolites (HETEs).
- The method is applicable for initial survey of lipoxygenases from animal sources.
- HETEs are separated on reversed-phase column and identified on straight-phase.
- This enables the separation of 12-HETE and 15-HETE to the baseline.
- The method was verified on rat lung cytosolic lipoxygenase.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Peter Hoffman, DrahomÃra Rauová, Lýdia Bezáková, Marek Obložinský, Peter MikuÅ¡,