| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7632146 | Journal of Pharmaceutical and Biomedical Analysis | 2013 | 10 Pages |
Abstract
⺠In vitro/in vivo metabolism investigation of combretastatin A-1. ⺠LC-UV and LC-MS experiments. ⺠Synthesis and spectroscopic characterization of the quinone metabolite Q1. ⺠Cytotoxicity and antitubulinic activities of combretastatins quinone metabolites. ⺠Chemical reactivity toward tubulin (Ellman's assay).
Keywords
GTPN-ethyl-maleimideRLMTRISHLMN-acetyl-l-cysteineDTNBGSHLC–MS analysisNAC(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide5,5′-dithiobis(2-nitrobenzoic acid)guanosine-5′-triphosphateMTTTris(hydroxymethyl)aminomethanetubulinminimal essential mediumRetention timeMEMReactive metabolitesRat liver microsomesHuman Liver MicrosomesNEMreduced glutathioneQuinones
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Silvio Aprile, Roberta Zaninetti, Erika Del Grosso, Armando A. Genazzani, Giorgio Grosa,