| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7633992 | Journal of Proteomics | 2017 | 51 Pages |
Abstract
Experimental workflow. Proteins were isolated from embryogenic tissue (E), non-embryogenic callus (NEC) and embryogenic tissue after loss of maturation capacity (E-L) of two genotypes (366, 362), analyzed by two-dimensional protein electrophoresis (2-DE) in biological triplicate. A. PDQuest analysis of 2-DE gels (comparison of embryogenic tissue and non-embryogenic callus) resulted in 24 differentially abundant proteins common for both cell lines 362 and 366. B. PDQuest analysis of 2-DE gels (comparison of embryogenic tissue and embryogenic tissue after loss of maturation capacity) resulted in 2 differentially abundant proteins common for both cell lines (up: more accumulated proteins, down: less accumulated proteins).181
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
KatarÃna Klubicová, Lubica Uvácková, Maksym Danchenko, Peter Nemecek, LudovÃt Skultéty, Ján Salaj, Terézia Salaj,