Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10Â days; comparison with animals exposed to morphine and subsequently nurtured for 20Â days in the absence of this drug

Article ID	Journal	Published Year	Pages	File Type
7634596	Journal of Proteomics	2016	13 Pages	PDF

Keywords

PBS heterotrimeric guanine nucleotide-binding regulatory proteins w.w.MMTS Na, K-ATPase MALDI-TOF MS/MS SLB LFQ GPCR PMSF IEF CBB TFA TCA ACN NaCl ABC SDS DOC DTT FDR 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate Coomassie Brilliant Blue DAMGO G-protein-coupled receptor LC–MS/MS tris(2-carboxyethyl)phosphine δ-Opioid receptor μ-Opioid receptor adenylyl cyclase EDTA ethylenediamine-tetraacetic acid Acetonitrile matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Trifluoroacetic acid trichloroacetic acid Electrophoresis two-dimensional gel electrophoresis TEAB Ammonium bicarbonate isoelectric focusing DADLE CNS dithiothreitol TCEP sodium dodecyl sulfate Sodium deoxycholate Sodium chloride central nervous system Liquid chromatography mass spectrometry Plasma membrane phenylmethylsulfonyl fluoride Long-term exposure Phosphate-buffered saline Label-free quantification morphine false discovery rate wet weight G proteins post-nuclear supernatant CHAPS PNS HPLC high-performance liquid chromatography κ-opioid receptor

Related Topics

Physical Sciences and Engineering Chemistry Analytical Chemistry

Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10Â days; comparison with animals exposed to morphine and subsequently nurtured for 20Â days in the absence of this drug

Authors

Hana Ujcikova, Miroslava Vosahlikova, Lenka Roubalova, Petr Svoboda,