Article ID Journal Published Year Pages File Type
7634777 Journal of Proteomics 2016 15 Pages PDF
Abstract
In this study, we monitored the light-induced transformation of prolamellar bodies into thylakoid membranes, which is correlated to the biogenesis of photosynthetic apparatus involving a complex cascade of biochemical and structural events. Three stages of thylakoid development classified according to the thylakoid development status (Adam et al., 2011) were studied for biogenesis of photosynthetic apparatus: Stage 1, prothylakoids emerge from the disrupted PLBs; Stage 2, prothylakoids converted into primary thylakoids which were dispersed in the stroma; Stage 3, the continuous grana and stroma thylakoids are formed. The development stage-dependent changes in the proteomic profile of the thylakoids were analyzed by two-dimensional electrophoresis (2-DE). This information was complemented with the steady-state 77 K chlorophyll fluorescence of thylakoids at the corresponding development stage. Together, these analyses allowed us to further understand the molecular processes connected to the formation of functional thylakoid membranes.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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