Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7634777 | Journal of Proteomics | 2016 | 15 Pages |
Abstract
In this study, we monitored the light-induced transformation of prolamellar bodies into thylakoid membranes, which is correlated to the biogenesis of photosynthetic apparatus involving a complex cascade of biochemical and structural events. Three stages of thylakoid development classified according to the thylakoid development status (Adam et al., 2011) were studied for biogenesis of photosynthetic apparatus: Stage 1, prothylakoids emerge from the disrupted PLBs; Stage 2, prothylakoids converted into primary thylakoids which were dispersed in the stroma; Stage 3, the continuous grana and stroma thylakoids are formed. The development stage-dependent changes in the proteomic profile of the thylakoids were analyzed by two-dimensional electrophoresis (2-DE). This information was complemented with the steady-state 77Â K chlorophyll fluorescence of thylakoids at the corresponding development stage. Together, these analyses allowed us to further understand the molecular processes connected to the formation of functional thylakoid membranes.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yangyang Wang, Kuixian Ji, Shihua Shen, Hui Chen,