| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7637745 | Journal of Proteomics | 2013 | 13 Pages |
Abstract
⺠We benchmark three of the most popular MS based quantification strategies. ⺠Including metabolic and chemical labeling with MS and MS/MS based quantification ⺠TMT requires MS3 based acquisition to overcome co-isolation at the cost of efficiency. ⺠SILAC and chemical labeling perform equal, independent of search algorithm used.
Keywords
CIDIsobaric labelingAP-MSPSMSAGCITRAQTMTNCEHCDFDRMS/MSscxnormalized collision energyisobaric tag for relative and absolute quantificationTandem mass tagDimethyl labelingstrong cation exchangecollision induced dissociationSILACTandem mass spectrometryfalse discovery ratestable isotope labeling by amino acids in cell cultureQuantitationautomatic gain control
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
A.F. Maarten Altelaar, Christian K. Frese, Christian Preisinger, Marco L. Hennrich, Andree W. Schram, H.Th. Marc Timmers, Albert J.R. Heck, Shabaz Mohammed,