| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7638248 | Journal of Proteomics | 2013 | 19 Pages |
Abstract
⺠New strategy for protein ID from micro areas based on microextraction is presented. ⺠Extraction areas are defined according to clustering of MALDI MSI data. ⺠1500 proteins are identified from 650 μm diameters area (about 1900 cells). ⺠ID proteins are back correlated to MALDI MSI data thanks to high mass accuracy.
Keywords
MSIEtOHppmLCMETDROITOFFDRLTQFWHMANIISDHCCARPLCDESILESACIDliquid extraction surface analysisSSSPDBSBSAFFPENd:YAGPCAS/Nbovine serum albuminAnilineEthanolMicro-extractionα-cyano-4-hydroxycinnamic acidSinapinic acidElectron transfer dissociationHESIndium tin oxideITOpart per millionPrincipal component analysismass spectrometry imagingcollision induced dissociationLinear ion trapHierarchical clusteringTime-of-Flight Signal to noiseIdentificationProtein identificationfull width at half maximumlaser capture microdissectionMALDI MSIMethanolregion of interestMeOHfalse discovery rateatomic mass unitShotgun proteomicsReverse phase liquid chromatographyNeodymium-doped yttrium aluminum garnetdesorption electrospray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jusal Quanico, Julien Franck, Claire Dauly, Kerstin Strupat, Jocelyn Dupuy, Robert Day, Michel Salzet, Isabelle Fournier, Maxence Wisztorski,