| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7638402 | Journal of Proteomics | 2012 | 9 Pages |
Abstract
⺠We developed a novel method - iMASp (identification of Mutated And Secreted proteins). ⺠Human Protein Mutant Database (HPMD) has 171,919 known disease mutations and SNPs. ⺠We detected 112 protein mutations from 18 colorectal cancer cell line secretome. ⺠Protein mutations identified by MS were verified by RT-PCR and cDNA sequencing. ⺠Three mutations were common between colorectal tissue and cell line secretome.
Keywords
HNPCCFAPSRMAPCITSSNPsPSACeAadenomatous polyposis colicarcinoembryonic antigenplasma biomarkersCulture mediaColorectal cancerHereditary Nonpolyposis Colorectal Cancerlactate dehydrogenaseLDHselected reaction monitoringprostate-specific antigenSingle nucleotide polymorphismsfamilial adenomatous polyposisCRC
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Suresh Mathivanan, Hong Ji, Bow J. Tauro, Yuan-Shou Chen, Richard J. Simpson,