Article ID Journal Published Year Pages File Type
7639813 Microchemical Journal 2018 9 Pages PDF
Abstract
The Breast cancer is the second most common cancer in women. So early detection of cancer is very important. We prepared a novel electrochemical genosensor for label-free, sensitive and selective detection of BRCA1 gene sequence. The developed sensor capture probe (DNA-p) hybridize to target BRCA1 gene sequence (DNA-t) on electrospinned ribbon conductive nanofibers (RCNFs) nanofiber electrospinned ribbon conductive nanofibers of polyethersulfone and nanotubes (RCNFs - MWCNTs) modified carbon paste electrode. This platform has a conductive and high mechanical strength and the surface to electrode volume ratio has increased 2 times. The construction of the platform for the sensor is very simple and cost-effective and the nanofiber can be spun in one step on the electrode surface. The BRCA1 gene sequence could be specifically recognized by capture aptamer pre-immobilized on modified carbon paste surface. Under optimum conditions, the electrochemical impedance spectroscopy (EIS) signals were proportional to the BRCA1 gene sequence concentration from 5 to 14,000 pM with a detection limit of 2.4 pM. The genosensor also exhibited high selectivity, stability and reproducibility with recoveries between 101.5 and 105.2%, indicating the developed biosensor would be a potential alternative tool for BRCA1 gene sequence detection in real biological samples.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , ,