Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7640076 | Microchemical Journal | 2018 | 26 Pages |
Abstract
α-Dicarbonyl compounds, such as 3-deoxyglucosone (3-DG), glyoxal (Gly), methylglyoxal (MGly), diacetyl (DA), and pentane-2,3-dione (PD) have been widely studied because they are the main precursors of advanced glycation end products. In this work, an analytical procedure was developed for the determination of these compounds by HPLC-UV using 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine (DQB) as a novel derivatizing reagent. After derivatization at pHâ¯9.0 and ambient temperature for 30â¯min, these five compounds were analyzed by HPLC. The results showed that the linear correlation coefficients (R2) of the method for the five analytes are between 0.994 and 0.999 in broad concentration ranges, the limits of detection are 0.050â¯Î¼M (3-DG), 0.050â¯Î¼M (Gly), 0.050â¯Î¼M (MGly), 0.020â¯Î¼M (DA), and 0.025â¯Î¼M (PD), respectively. The method was further evaluated in the analyses of many kinds of food samples employing the standard addition method with the RSDs ranging from 0.43 to 4.07% and the recoveries from 85% to 103%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xin-Jie Wang, Feng Gao, Lu-Chao Li, Xian Hui, Heng Li, Wen-Yun Gao,