Article ID Journal Published Year Pages File Type
7640522 Microchemical Journal 2018 9 Pages PDF
Abstract
In this study, Super Critical CO2 (SCC) was applied to extract the active ingredients from Multi-herbal formula Chaihu Shugan San (CSS). Response Surface Methodology (RSM) coupled with an improved chaotic Gray Wolf Optimization (ACGWO) was employed to the experimental design. After gas chromatography-mass spectrometer (GC-CI/EI-MS) detection, liquid chromatography-mass spectrometry- ion trap-time of flight (LC-MS-IT-ToF) further indicated that several phthalides are mainly responsible for the SCC extract; the next is α-Cyperone in Cyperus rotundus; and Meranzin hydrate, Isomeranzin in Citrus reticulata Blanco again. Further pharmacological tests were also performed for the SCC extracts. THP-1 macrophages were used to observe the anti-inflammatory effects. The expression of IL-6 was obviously suppressed by the SCC extract from CSS, through a positive correlation with the dose. Then, ROS content in a cell model was used to detect the antioxidant capacities. The results showed that the SCC extract from CSS, exert dose-dependent inhibitory effects on the oxidative stress of the cells.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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