Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7640726 | Microchemical Journal | 2018 | 24 Pages |
Abstract
A new environmental friendly methodology for metsulfuron-methyl quantification based on the fluorescent signal enhancement of rhodamine B dye has been developed. A cationic surfactant (cetyltrimethylammonium bromide, CTAB) and an anionic one (sodium dodecylsulfate) were employed to preconcentrate the herbicide using a coacervation phenomenon, in sodium borate buffer medium (pH 9.2). The coacervate phase was collected on a nylon membrane (0.45 μm) and the solid surface fluorescence signal was determined (λexc = 515 nm, λem = 565 nm). Experimental variables that influence on preconcentration step and fluorimetric sensitivity have been studied and optimized using response surface methodology. Under optimal working conditions, a LOD of 0.17 μg Lâ1 and a LOQ 0.53 μg Lâ1 was obtained. The zeroth order regression calibration was linear from 0.53 to 5.00 μg Lâ1. The method showed adequate sensitivity and selectivity, and was applied to the determination of trace amounts of metsulfuron-methyl in environmental water samples. The proposed methodology implies an alternative to traditional techniques for metsulfuron-methyl monitoring using an accessible instrument in control laboratories, representing a contribution in the toxicological and environmental areas for the monitoring of MSM in environmental samples, in agreement with the Green Chemistry.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Magdalena Alesso, César A. Almeida, MarÃa C. Talio, Liliana P. Fernández,