Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7641341 | Microchemical Journal | 2017 | 8 Pages |
Abstract
We have established an isotope labeling protocol for determining parabens in wastewater, based on a pair of isotope-coded derivatization reagents, by high performance liquid chromatography (LC) and electrospray mass spectrometry (ESI-MS). The pair of reagents used in our work were d0-10-methylacridone-2-sulfonyl chloride (d0-MASC, light form) and d3-10-methylacridone-2-sulfonyl chloride (d3-MASC, heavy form). d0-MASC and d3-MASC could easily derivatize parabens at 55 °C for 7.5 min in the mixture of bicarbonate and acetonitrile (1:1 v/v). It is shown that the derivatization offers 1-2 orders of magnitude signal enhancement over underivatized counterparts. The global isotope internal standard technology was employed for quantification analysis with d3-MASC-paraben as internal standard for corresponding d0-MASC-paraben. In addition, homologous internal standard was simultaneously used to correct derivatization processes. The obtained double internal standard calibration curves for parabens were linear over the range of 1-500 nmol Lâ 1 (r > 0.997). The isotope-coded derivatization based quantification method was precise and practical with RSD â¤Â 3.6% and accuracy ranged 96.5 to 104.7%. The proposed method has also been successfully applied to the quantification of parabens in domestic sewage samples with precisions â¤Â 5.2% and recoveries â¥Â 87.9%. Five kinds of paraben including methylparaben, ethylparaben, propylparaben, butylparaben, and benzylparaben were detected in the tested samples. The quantitative results indicated that parabens were at nmol Lâ 1 level or lower, and ethylparaben were the most abundant, followed by methylparaben, propylparaben and butylparaben. It has been demonstrated that the isotope-coded derivatization reagents are useful for accurate quantification of analytes having target functional groups in real samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dandan Che, Zhiwei Sun, Jie Cheng, Kun Dou, Zhongyin Ji, Guang Chen, Guoliang Li, Jinmao You,