Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7642253 | Microchemical Journal | 2016 | 4 Pages |
Abstract
In this study a gold electrode was used to indirect determination of melittin in nonaqueous medium by square wave stripping voltammetric (SWSV) method. The method is based on measuring the decrease of oxidation peak current of ferrocene with addition melittin. The optimum conditions include an accumulation potential of 0Â mV (versus Ag/Ag+), an accumulation time of 10Â s. A calibration curve was obtained for melittin in a linear range of 40 to 240Â nM with a low detection limit (LOD) of 12Â nM (at a signal-to-noise ratio of 3). The validated SWSV method showed good linearity as well as satisfactory repeatability and immediate precision values, for both instrument and method. The method was successfully applied to the determination of melittin in honeybee venom sample.
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Authors
Ummihan Taskoparan Yilmaz, Tugba Melekogullari, Aysel Kekillioglu, Demet Uzun,