Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7643028 | Microchemical Journal | 2014 | 4 Pages |
Abstract
Psoriasis is a T lymphocyte-mediated inflammatory disorder that affects the skin. A number of studies have demonstrated the occurrence of lipid alterations in psoriatic skin, resulting in a highly perturbed stratum corneum (SC). Relatively little attention has been paid to the protein conformation of the SC. In this study, the attenuated total reflection Fourier transform infrared (ATR-FTIR) spectrum of the untreated psoriatic patients' unharmed SC was obtained after tape stripping. We focused on the amide-I band components in order to establish whether there are any protein alterations in the intact areas of psoriatic skin. Fourier self-deconvolution (FSD) of the amide-I band was followed by curve-fitting to generate the underlying components. Integration of band areas provided an estimate of the secondary structure. The results indicated decreases in all amide-I band components, the peak at 1660 cmâ 1 revealing the most dramatic change. This peak is characteristic of the turn structure in the protein chain. The decrease is marked in the case of the β-sheet structure at 1630 cmâ 1 too. This ATR-FTIR imaging is a rapid and simple noninvasive method, promotes a better understanding of the disease, and would be helpful in following the treatment.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Boglárka Balázs, Gabriella Farkas, Ottó Berkesi, Rolland Gyulai, Szilvia Berkó, Mária Budai-Szűcs, Piroska Szabó-Révész, Lajos Kemény, Erzsébet Csányi,