Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7652685 | Revue Francophone des Laboratoires | 2013 | 4 Pages |
Abstract
Amphotericin B is an effective antifungal drug used in the treatment of systemic mycosis, but it has a narrow therapeutic interval and an important renal toxicity. The aim of this study is to monitor and valid a new method for Amphotericin B quantitation in plasma using HPLC with UV/visible detection. A liquid-liquid extraction was done; thereafter, a chromatographic separation was achieved using an RP 18 column (125 mm). The mobile phase is composed of water, acetonitrile and acetic acid (51/44/5) (v/v/v), pH 3. Piroxicam is used as an internal standard. This technique is linear over the range 0,125 μg/mL to 8 μg/mL (r = 0,99). Detection and quantification limits were 0.044 μg/mL and 0.111 μg/mL, respectively. Within-day coefficient of variation (1,98 to 4,66 %) and day-to-day coefficient of variation (2,69 to 4,95 %) were determined at three different concentrations. Under these conditions, each analysis requires no longer than 10 min.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Emna Gaïes, Ridha Ben Ali, Sameh Trabelsi, Nadia Jebabli, Issam Salouage, Rim Charfi, Mohamed Lakhal, Anis Klouz,