| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7661985 | Revue Francophone des Laboratoires | 2008 | 4 Pages |
Abstract
Hypoglycemia crisis of unknown origin can be induced by factitious sulfonylurea ingestion which as been described as Munchausen syndrom. It is essentiel to distinguish from an insulin-secreting tumor, and diagnostic can be done by using a method for identification of sulfonylureas in human plasma. Different analytical techniques have been used : most methods are based on HPLC with ultra-violet, or diode array which is more specific. More recently, highly selective liquid chromatography-electrospray tandem mass spectrometry (MS-MS) has been described for rapid screening. We report the case of hypoglycemic crises in a 38 years old woman. HPLC-DAD was used after liquid/liquid extraction of plasma and urine samples. Identification of gliclazide was done in three plasma samples but not in urine. This HPLC-DAD assay can be highly convenient but need plasma sampling at a time period closed from hypoglycemic crise.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Christophe Bardin, Naïma Tafzi,