Design and synthesis of a novel lanthanide fluorescent probe (TbIII-dtpa-bis(2,6-diaminopurine)) and its application to the detection of uric acid in urine sample

A novel fluorescent probe, Tb-dtpa-bdap, is designed based on the principle of complementary base pairing and synthesized for uric acid detection. The affecting factors, including solution pH, uric acid concentration and interfering substances, on the detection of uric acid using this prepared probe are examined. Under the optimized conditions, the fluorescence intensities of Tb-dtpa-bdap solution towards different uric acid concentrations show a linear response in the range from 1.00 × 10−5 mol·L−1 to 5.00 × 10−5 mol·L−1 with the correlation coefficient (R2) of 0.9877. And the obtained limit of detection (LOD) is about 5.80 × 10−6 mol·L−1, which is much lower than the level of uric acid in actual urine. The mechanism on the detection of uric acid by using Tb-dtpa-bdap is inferred according to the above mentioned experimental results. The facts demonstrate that the proposed fluorescent probe may be successfully applied for the determination of uric acid in human urine samples.267