A new water-soluble polythiophene derivative as a probe for real-time monitoring adenosine 5′-triphosphatase activity in lysosome of living cells

The real-time assays for ATPase activity in lysosome of living cells were achieved through a fluorescence "turn-on" mode by taking advantages of the reaction specificity of ATPase with ATP and the high binding selectivity of the probe toward ATP substrate over its hydrolysis product (ADP).268