Sensitive and specific detection of microRNAs based on two-stage amplification reaction using molecular beacons as turn-on probes

In this study, two MBs were designed. MB I, with nine flanking base pairs in its stem region, was used for real-time monitoring of the production of Y during the TSAR ( 1). MB II, with five flanking base pairs in its stem region, was used to detect the production of Y at the end of the TSAR ( 2).131