Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7678560 | Talanta | 2015 | 5 Pages |
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn2+ quantum dots (CdTe:Zn2+ QDs) fluorescence by Stern-Volmer constants nearly 109Â Mâ1 without any aggregation, the CdTe:Zn2+ QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn2+ QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4Â ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lu Chen, Shiyi Ye, Kai Cai, Cuiling Zhang, Guohua Zhou, Zhike He, Heyou Han,