Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7679576 | Talanta | 2015 | 5 Pages |
Abstract
The fabrication of Fe3O4@Au composites as a novel resonance light scattering (RLS) probe for the sensitive detection of Alzheimer׳s amyloid-β peptide (Aβ) was demonstrated. Amino groups coated magnetic Fe3O4 nanoparticles were covered with gold shell by the classical Frens method. The resultant colloids were characterized with X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS) and UV-visible spectra. The results indicated that the composite particles with core/shell structure and an average diameter of ~320 nm were stable and biocompatible. The RLS intensity of Fe3O4@Au composites was significantly enhanced by interacting with Aβ. Under optimal conditions, good linear relationship between the ratio of RLS intensity I/I0 at 463.0 nm and the logarithmic value of Aβ concentration in the range of 5.0Ã10-15-5.56Ã10â9 M was found. The limit of detection (LOD) was 1.2Ã10â15 M. The proposed method is simple, sensitive and cost-effective and complementary to other existing methods for protein analysis.
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Authors
Ling Yu, Yintang Zhang, Ran Chen, Danhua Zhang, Xiuhua Wei, Fang Chen, Jianxiu Wang, Maotian Xu,