Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7681918 | Talanta | 2013 | 8 Pages |
Abstract
We have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22 kDa (22K) and 20 kDa (20K). An oriented-antibody sensor surface specific for both hormone isoforms was assembled by using the biotin-streptavidin system. The immunosensor functionality was checked for the direct detection of the 22K hGH isoform in buffer, which gave high specificity and reproducibility (intra and inter-assay mean coefficients of variation of 8.23% and 9% respectively). The selective determination of the 22K and 20K hGH isoforms in human serum samples in a single assay was possible by using two specific anti-hGH monoclonal antibodies. The detection limit for both hormone isoforms was 0.9 ng mLâ1 and the mean coefficient of variation was below 7.2%. The excellent reproducibility and sensitivity obtained indicate the high performance of this immunosensor for implementing an anti-doping test.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Elena de Juan-Franco, J.M. RodrÃguez-Frade, M. Mellado, Laura M. Lechuga,