Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7682557 | Talanta | 2013 | 4 Pages |
Abstract
Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10â²-phenothiazinyl)propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09 pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5 ng mLâ1. The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ivan Yu. Sakharov, Alexandra S. Demiyanova, Anastasia V. Gribas, Natalia A. Uskova, Evgeny E. Efremov, Marina M. Vdovenko,