Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7682581 | Talanta | 2013 | 5 Pages |
Abstract
In this paper unmodified gold nanoparticles (AuNPs) were used as a sensing element to detect ramoplamin. Detection relies on the fact that the dispersed AuNPs solution is red due to the intense surface plasmon absorption band at 530Â nm whereas the AuNPs solution in the presence of ramoplanin is blue. Upon aggregation, there is a significant change in absorbance intensity at 620Â nm. Based on the aggregation of AuNPs induced by ramoplanin, a simple colorimetric method was developed for determining the of ramoplanin concentration. Experimental conditions influencing the analytical performance such as particle size, amount of AuNPs, incubation time and pH were evaluated. Under the optimized experimental conditions, this method could detect ramoplanin in a linear range from 0.30 to 1.30Â ppm with a detection limit of 0.01Â ppm and exhibited good reproducibility, selectivity and recovery. Analysis time of this assay was only 2Â min. To investigate its potential applicability, this assay was successfully applied for the determination of ramoplanin in urine samples without costly instruments.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Siriwan Teepoo, Phongnarin Chumsaeng, Khwankhao Palasak, Natvara Bousod, Naree Mhadbamrung, Phorntip Sae-lim,