Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7682668 | Talanta | 2013 | 17 Pages |
Abstract
This paper reports the application of a chiral imprinted polymer (CIP)-coated stir bar for the selective extraction of (+)-(S)-citalopram (SCIT) and its main metabolites, (+)-(S)-desmethylcitalopram (SDCIT) and (+)-(S)-didesmethylcitalopram (SDDCIT), from urine samples. The developed device has been demonstrated to be capable of selectively extracting the three target analytes from urine samples without saturating the imprinted sites. A CIP-coated stir bar sorptive extraction procedure (CIP-SBSE) is proposed for the isolation of SCIT, SDCIT and SDDCIT followed by their subsequent analysis using liquid chromatography ion trap mass spectrometry (LC-ITMS). Deuterated SCIT-d6 was used as an internal standard. The method was validated using a standard procedure, which revealed that a quantification of 5 ng mLâ1 was obtained in urine samples and that the accuracy and precision were within the established values while no matrix effect was observed.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nora Unceta, Alberto Gómez-Caballero, Deiene GarcÃa, Goretti DÃaz, Antonio Guerreiro, Sergey Piletsky, M. Aránzazu Goicolea, Ramón J. Barrio,