Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7682885 | Talanta | 2013 | 6 Pages |
Abstract
A newly designed functionalization type for gold nanoparticles (AuNP) with split aptamer has been developed for the detection of adenosine triphosphate (ATP). The ATP aptamer was split into two parts with their 5â² prime or 3â² prime modified with thiol. Both the 5â² SH and 3â² SH modified strands for each split aptamer fragment were functionalized onto the same AuNP to construct double-functionalized AuNP-DNA conjugates. Thus, the split aptamer can be reassembled into intact folded structure in the presence of ATP molecule with two potential assembly types, which induces the assembly of AuNP-DNA conjugates. In this double-functionalized system, the traditional assembly type might facilitate another assembly type, which was found to give much higher LSPR change in the presence of ATP than the traditional assembly type, and improve the sensitivity for ATP detection. Time courses of the assemble processes with different assembly types, Mg2+ concentrations, and aptamer fragments densities on AuNP were followed using the absorption ratio at 650Â nm and 520Â nm. ATP response with this newly designed system was investigated using absorption spectra and dynamic light scattering method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sheng Cheng, Bin Zheng, Mozhen Wang, Michael Hon-Wah Lam, Xuewu Ge,