Development of a new gas chromatography-mass spectrometry (GC-MS) methodology for the evaluation of 5Î±-reductase activity

Article ID	Journal	Published Year	Pages	File Type
7683441	Talanta	2013	8 Pages	PDF

Abstract

âº This work presented a new DLLME-GC/MS method to quantify T and DHT. âº This method evaluate the 5Î±-reductase activity in human prostate microsomes. âº Present a good linearity, good extraction efficiency, accuracy and high recovery. âº Avoids the use of radioactive compounds and needs less sample for the enzymatic reaction. âº The method can be applied to study the biochemical activity of new potential RIs.

Keywords

BSTFA C2Cl4 N-methyl-N-(trimethylsilyl)trifluoroacetamide LC/APCI-MS metanol MECN MSTFA HFBA 5α-R DLLME RIS DTE TLC NADPH DTT DHT 1,1,1-trichloroethane 1,4-dithioerythritol 5α-reductase DMSO GC–MS LC/ESI-MS Dispersive liquid–liquid microextraction (DLLME)Dispersive liquid–liquid microextraction Tetrachloroethylene Heptafluorobutyric anhydride Acetonitrile Trichloroethylene testosterone Dihydrotestosterone dithiothreitol Dimethyl sulfoxide SIM gas chromatography–mass spectrometry Gas chromatography–mass spectrometry (GC–MS)5α-reductase inhibitors MeOH selected ion monitoring BPH Benign prostate hyperplasia Prostate thin layer chromatography high performance liquid chromatography HPLC Chlorobenzene Androgen Receptor Ammonium iodide electron ionization