At-line NIR spectroscopy as effective PAT monitoring technique in Mab cultivations during process development and manufacturing

► NIRS on supernatant samples from Mab cultivation processes spanning several production scales, CHO cell lines and development years. ► NIRS is effective in replacing in-process monitoring of some critical process parameters. ► NIRS enables to establish batch trajectories and investigate deviations from nominal operational conditions. ► The proposed strategy is applicable throughout process development and large scale validated CHO bioprocesses.