Electrochemical immunosensor for competitive detection of neuron specific enolase using functional carbon nanotubes and gold nanoprobe

► Using SWNT to enhance electrochemical signal and enlarge antigen domains. ► AP linked secondary antibody labeled AuNP enhanced catalytic activity to α-NP. ► Using SWNT and AuNP lead to a dual signal amplification for sensitive detection. ► The sensor with a wide range can direct detect protein in serum without dilution. ► The assay results were in a good agreement with the reference values.