Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7689576 | TrAC Trends in Analytical Chemistry | 2015 | 23 Pages |
Abstract
A genetically modified organism (GMO) is defined as a living organism whose genome has been modified by the introduction of an exogenous gene able to express an additional protein that confers new characteristics, such as enhancement of the nutritional properties, herbicide resistance or insect protection. The need to monitor and to verify the presence and the amount of GMOs in agricultural crops and in food products has generated interest in analytical methods for accurate, sensitive, rapid, cheap detection of these products. A novel DNA-detection technology was developed: genosensors. This article reviews electrochemical DNA biosensors reported for the qualitative and quantitative determination of transgenic traits. We discuss critical aspects of genosensor design with particular emphasis on analytical characteristics and analysis of real samples.
Keywords
SAMRT-PCRGDLCNTssDNADPASVHMGAPolypyrroleHRPCPEAuNPPDCSPEdsDNAASVMWCNTSWCNTPPyPDDATMBPhosphinothricin acetyltransferase geneMCHGATPEPSPCEGMOp-AminothiophenolEPSPsEDCGCENOSDPVFITCSWV2,6-pyridinedicarboxylic acidSingle-stranded DNAdouble-stranded DNADNAPANIGlucose oxidasegenetically modified organismdeoxyribonucleic acidScreen-printed gold electrodescreen-printed electrodeCarbon-paste electrodeScreen-printed carbon electrodeglassy carbon electrodeSelectivityGoxElectrochemical DNA biosensortetramethylbenzidineEISEnzyme-linked immunosorbent assayELISADNA detectionSelf-assembled monolayerDetection methodnopaline synthaseElectrochemical impedance spectroscopyfluorescein isothiocyanateMethylene blueFood productMercaptohexanolGold nanoparticleCarbon nanotubeMulti-walled carbon nanotubepolymerase chain reactionreal-time polymerase chain reactionPCRAnodic stripping voltammetryDifferential pulse voltammetryCyclic voltammetrySquare Wave VoltammetrypatHorseradish peroxidasePoly(diallyldimethyl ammonium chloride)PolyanilineElectrochemical genosensorGluGlucosegluconolactone
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
C. Lorena Manzanares-Palenzuela, Begoña MartÃn-Fernández, Marta Sánchez-Paniagua López, Beatriz López-Ruiz,