Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7690100 | TrAC Trends in Analytical Chemistry | 2014 | 12 Pages |
Abstract
Liquid chromatography-mass spectrometry (LC-MS) is one of the most prominent analytical techniques, due to its inherent selectivity and sensitivity. In LC-MS, chemical derivatizations are frequently used to enhance the MS ionization efficiency and selectivity, to facilitate structure elucidation, and to improve the chromatographic separation. In this review, we present an overview of derivatization-based LC-MS analysis. We summarize the reaction mechanisms of representative derivatization reagents and the selection strategy to guide and to stimulate future studies. Furthermore, we emphasize applications of derivatization in peptide and protein analysis, metabolite analysis, environmental analysis, pharmaceutical analysis, food-safety evaluation and MS imaging.
Keywords
SFPMSIITRAQFMEAAPDSFmoc-ClDEEMMMSTFANBD-FSBD-F3-NitrophenylhydrazinePFBBrNQS1,2-naphthoquinone-4-sulfonateDANSYL hydrazine2-nitrophenylhydrazinem-Aminophenylboronic acidN-(3-dimethylaminopropyl)-N′-ethylcarbodiimidep-BPBDabsyl chlorideDNS-ClBPBAESIDNPHEDCLC-MSICATDMBACIDHMPBPb2,4-dinitrophenylhydrazine9-fluorenylmethyl chloroformatePMAp-Bromophenacyl bromideCollision-induced dissociationisobutyl chloroformateBtAisobaric tags for relative and absolute quantificationPharmaceutical analysisEnvironmental analysisMass spectrometric imagingDansyl chloridediethyl ethoxymethylenemalonateLiquid chromatography-mass spectrometryMass spectrometryMetaboliteDerivatizationIAMisotope-coded affinity tagProteinPentafluorobenzyl bromidePeptideliquid chromatographyelectrospray ionizationiodoacetamide
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bao-Ling Qi, Ping Liu, Qiu-Yi Wang, Wen-Jing Cai, Bi-Feng Yuan, Yu-Qi Feng,