| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7692692 | Chemistry and Physics of Lipids | 2012 | 11 Pages |
Abstract
⺠PLPC oxidation was induced by H2O2 in the presence or absence of CuCl. ⺠Using hydrazine chemistry and ESI-MS 26 reactive oxidation products were identified. ⺠Reactivity of nucleophilic amino acids towards lipid-derived carbonyls was studied. ⺠10, 9 and 3 lipid-derived adducts were identified on Lys, Cys and His, respectively. ⺠Lys and Cys showed the highest reactivity towards PLPC-derived reactive carbonyls.
Keywords
UPLCLAPPPLPCMichael adductGlycerophosphatidylcholineelectrospray ionization mass spectrometry (ESI-MS)FDPGPCLPPCIDDNPH2,4-dinitrophenylhydrazineROSRPcEDTAEthylenediaminetetraacetic acidLinoleic acidSchiff basecollision induced dissociationRoom temperaturephosphatidylcholineLipid peroxidation productsFenton reactionProteomicsReversed phase chromatographyultra performance liquid chromatographyReactive oxygen species
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Ivana Milic, Maria Fedorova, Kristin Teuber, Jürgen Schiller, Ralf Hoffmann,