Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7692726 | Chemistry and Physics of Lipids | 2012 | 7 Pages |
Abstract
⺠Sphingosine-1-phosphate lyase (GPL) yields aldehydes from sphingoid phosphates. ⺠GPL produces pentadecanal from 2-amino-3-hydroxyheptadecyl phosphate (C17-dhS1P). ⺠GC/MS quantification of pentadecanal as its pentafluorobenzyloxime (C15-PFBO). ⺠GPL activity by incubation with C17-dhS1P and GC/MS analysis of C15-PFBO.
Keywords
DOPO-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloridePFBHA(Z)-11-hexadecenalFTY720S1PMEFPFBOSGPL1DHS1PGC/MSSphingosine-1-phosphate lyaseSphingosine-1-phosphatesphingolipidSphinganine-1-phosphateDetection limitSIMMass spectrometrymouse embryonic fibroblastsselected ion monitoringGas chromatography
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Ester Reina, Luz Camacho, Josefina Casas, Paul P. Van Veldhoven, Gemma Fabrias,