Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7692912 | Chemistry and Physics of Lipids | 2011 | 7 Pages |
Abstract
The simple and low cost β-cyclodextrin (β-CD)-phenolphthalein (PHP) inclusion complex was used for both the study of physical-chemical parameters and validation of analytical procedures for deoxycholic acid (DCA) and ursodeoxycholic acid (UDCA) determinations in different formulations. The usefulness of this inclusion complex is proposed either in the form of kit reagent and as an original optical sensor for DCA and UDCA. The results showed that temperature had a negative effect on the equilibrium constant resulting in high negative values of enthalpy and positive values of entropy. The half-life values for DCA and UDCA measurements were 68.71 and 294.71 days, respectively. The method was validated showing limits of detection and quantification of 4.92 Ã 10â5 mol Lâ1 and 1.64 Ã 10â4 mol Lâ1 for DCA, 1.14 Ã 10â5 mol Lâ1 and 3.79 Ã 10â5 mol Lâ1 for UDCA, respectively. The developed optical sensor also showed response linearity, ease of implementation and potential application in fast screening tasks even out of the laboratory.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Pabyton G. Cadena, Alberto N. Araújo, Maria C.B.S.M. Montenegro, Maria C.B. Pimentel, José L. Lima Filho, Valdinete L. Silva,