| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7694378 | Current Opinion in Chemical Biology | 2015 | 7 Pages |
Abstract
- Concerns about CRISPR specificity led to new methods for detection and avoidance.
- Some studies reported many off-target events, while others observed few.
- High or low specificity is a property of the guide RNA used, not CRISPR/Cas9 per se.
- The current challenge is to find the most specific guide RNA.
- The specificity determinants for Cas9 nuclease and dCas9 binding are different.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Henriette O'Geen, Abigail S Yu, David J Segal,